Campus Guarulhos • Escola de Filosofia, Letras e Ciências Humanas
Nombre del programa de postgrado

Artículo 1


Objective: 
As Selumetinib is a MEK1/2 inhibitor that has gained interest as an anti-tumor agent, the present study was designed to investigate autophagy involvement on Selumetinib-induced apoptosis in colorectal cancer (CRC) cells.

Methods: CRC cells death and cycle studies were assessed by AnnexinV-FITC and PI staining, respectively. Autophagy flux was analysed by Western Blot (LC3II and p62 protein levels) and retroviral infection of SW480 cells for siBecn1 RNA interference experiments. Confocal microscopy was used to determine mCherry-EGFP–LC3 distribution.

Key findings: The Selumetinib effects were concentration-dependent in SW480 cell line. Whereas 1 μM exerted an arrest in the cell cycle (G1 phase), higher concentrations (10 μM) induced cell death, which was accompanied by autophagy blockage in its last stages. Autophagy induction by Rapamycin (RAPA) increased cell survival, whereas pharmacology autophagy inhibition by Bafilomycin A1 (BAF), Chloroquine (CQ) or 3-Methyladenine (3-MA) increased Selumetinib-induced CRC cells death.

Conclusions: Altogether, these results suggest that autophagy plays a fundamental role in CRC cells response to Selumetinib. In addition, the combination of Selumetinib with autophagy inhibitors may be a useful therapeutic strategy to enhance its activity against colorectal tumours.

09 Sep 2020 19:46

Empatia - Edmund Husserl e Edith Stein

“Experiência da experiência alheia”, a empatia ou intropatia [ ... ]

Publicações
09 Sep 2020 20:05

Diálogos com Edith Stein
Filosofia, Psicologia, Educação

Este é um conjunto de quinze textos em [ ... ]

Publicações
09 Sep 2020 20:00

O Cinema Português Através dos Seus Filmes.

A obra consiste numa introdução crítica ao cinema [ ... ]

Publicações

 

© 2013 - 2024 Universidade Federal de São Paulo - Unifesp
Estrada do Caminho Velho, n° 333 - Jd. Nova Cidade - Guarulhos - SP - CEP: 07252-312
Telefone: (11) 5576-4848 (como ligar)

Mapa do CampusSobre o portal  |  Comunicar erros no site