The Research Laboratory in Otorhinolaryngol. (ORL Lab)
BRIEF HISTORY
The integration between clinical aspects and basic research has shown to be of fundamental importance for a better understanding of the etiopathogenesis of diseases, with promising results in improving the quality of life and survival of patients. In this context, the Department of Otorhinolaryngology and Head and Neck Surgery started in 2013 the implementation of a research laboratory. At first, the Otorhinolaryngology Research Laboratory (ORL Lab) had the basic function of serving as a repository of biological samples, cataloging and managing the dynamics of use of these materials. This attribution was quickly supplanted by actions aimed at training graduate students and carrying out new lines of research in the department.
The ORL Lab is currently linked to the Graduate Program in Medicine (Otorhinolaryngology), being managed by its professors. This fact gave this sector a multidisciplinary characteristic due to the lines of research developed, as well as the professional diversity of graduate students. As a result, several partnerships have been established with other UNIFESP laboratories as well as other universities and research institutions. This collaborative environment made this sector also become multicenter, allowing the use of its space, equipment and personnel by researchers and graduate students from other research centers. We currently have collaboration with the University of São Paulo (ICB, IF and FMUSP), Heart Institute (INCOR), University of Santo Amaro (UNISA), São Paulo University (UNIP), São Paulo Method Faculty (FAMESP), Brazilian Institute Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), School of Firefighters, Memorial Sloan Kettering Cancer Center, University of British Columbia-Vancouver and, at our institution, the Discipline of Nutrition Physiology, Lipid Sector, Atherosclerosis and Biology Vascular, Sports Medicine Center, Discipline of Geriatrics and Gerontology and, finally, all disciplines of the Department of Otorhinolaryngology and Head and Neck Surgery.
The result of all this work is expressed in numbers that confirm the growing improvement in the quality of research carried out and in the training of graduate students in the program. Since the installation of the ORL Lab in 2013, 16 master's dissertations and 12 doctoral theses have had their results produced in this sector. Finally, the production of scientific articles exemplifies all the aspects mentioned above. The 64 articles published until 2024 (See list of the last 5 years below) demonstrate that ORL Lab has become a sector that values collaborative work, being a place for professional growth and with an important role in the training of our graduate students.
The 3-Dimensional Cell Culture allows the development of in vitro biological systems that emulate characteristics found in animal models. These cell aggregates (also known as spheroids) allow a better understanding of aspects such as nutrient diffusion, drug resistance and cell selection. At the ORL Lab, both the nasal epithelium and cells derived from adenocarcinomas have been cultivated in 3D. The main objective of this line of research is to obtain an experimental platform that can be used to better understand the diseases associated with the upper airways, oral cavity and glands associated with them (More information).
Cellular and Molecular Aspects of Sensorineural Hearing Loss
Several studies have been carried out to clarify the etiopathogenesis of sensorineural hearing loss. There is no consensus, to date, on which factors are really important to predict the recovery of patients with this disease. Using different methodological approaches, the ORL Lab has described the role of different inflammatory cytokines and related cell signaling pathways in sensorineural hearing loss (More Information).
One of the lines of research at ORL Lab is the study of the mechanical dysfunction found in Nasal Polyposis, considering the immunological aspects, the remodeling process and the establishment of new mechanical models using cell culture (More Information).
Effect of Exposure to Heat and Combustion Derived Products on the Airway of Firefighters
Due to the increase in fires and fires in recent years and the inherent risks of thermal exposure and fire-derived products, we are developing studies in partnership with the Fire Department of the State of São Paulo to assess the inflammatory, immunological, endocrinological and impact on airways of individuals exposed to firefighting activities and their adaptive responses, in addition to proposing measures to mitigate its effects (More Information).
The evaluation of mucosal immune and inflammatory responses, especially in the upper airways, has gained prominence in recent times, because when making direct contact with the environment, the immunity generated in this location will prominently influence the protection against potentially pathogenic agents. Therefore, this line of research aims, in general, to investigate the modulations of immune and inflammatory responses present in the upper airways in different populations, with emphasis on the elderly, and situations such as, for example, the response to vaccination as well as the an exhausting exercise session, such as running a marathon (More Information).
Pezato R, Stamm AC, Douglas R, Han JK, Chen PG, Neto CDP, et al. Nasal disease with polyps: Need for clarity.International forum of allergy & rhinology. 2023;13(9):1829-31.
Pezato R, David AG, Boggi AC, Melo B, Rosalino CMV, Rodriguez AP, et al. Upper Airway Mucociliary Clearance is Impaired in Dyspneic COVID-19 Patients.Indian journal of otolaryngology and head and neck surgery: official publication of the Association of Otolaryngologists of India. 2023;75(2):772-6.
Do Amaral JB, David AG, Mello L, Bachi ALL, Voegels RL, Thamboo A, et al. Impact of Chronic Rhinosinusitis with Nasal Polyposis on IL-12 and IL-8.Iranian journal of otorhinolaryngology. 2023;35(126):21-7.
Silva BR, Monteiro FR, Cezário K, Amaral JBD, Paixão V, Almeida EB, et al. Older Adults Who Maintained a Regular Physical Exercise Routine before the Pandemic Show Better Immune Response to Vaccination for COVID-19.International journal of environmental research and public health. 2023;20(3).
Rosseto-Welter EA, Rodrigues SS, de Figueiredo AB, França CN, Oliveira DBL, Bachi ALL, et al. Cellular and Humoral Immune Responses to Vaccination for COVID-19 Are Negatively Impacted by Senescent T Cells: A Case Report.Vaccines. 2023;11(4).
De Carvalho DC, Fonseca FAH, Izar MCO, Silveira A, Tuleta ID, do Amaral JB, et al. Monocytes presenting a pro-inflammatory profile persist in patients submitted to a long-term pharmacological treatment after acute myocardial infarction. Frontiers in physiology. 2022; 13:1056466.
Aires MM, de Oliveira KVG, do Amaral JB, Mônico-Neto M, Martins JRM, Santiago AL, et al. Effect of Testosterone on the Thyroarytenoid Muscle and Lamina Propria of Female Rat Vocal Folds.The Laryngoscope. 2023.
Da Silva LS, Germano DB, Fonseca FAH, Shio MT, da Silva Nali LH, Tuleta ID, et al. Persistence of a proinflammatory status after treatment of the acute myocardial infarction.Geriatrics & gerontology international. 2023;23(9):700-7.
Santos CAF, Amirato GR, Paixão V, Almeida EB, Do Amaral JB, Monteiro FR, et al. Association among inflammaging, body composition, physical activity, and physical function tests in physically active women.Frontiers in medicine. 2023; 10:1206989.
Germano DB, Oliveira SB, Bachi ALL, Juliano Y, Novo NF, Bussador do Amaral J, et al. Monocyte chemokine receptors as therapeutic targets in cardiovascular diseases.Immunology letters. 2023;256-257:1-8.
De Góes HAN, Sarafan M, do Amaral JB, Dos Anjos Almeida J, Voegels RL, de la Cruz LT, et al. Differential Angiogenic Induction Impacts Nasal Polyp Tissue Growth.Indian journal of otolaryngology and head and neck surgery: official publication of the Association of Otolaryngologists of India. 2023; 75(Suppl 1): 893-900.
Nardy A, Camargo CTS, Oliveira YFC, Silva FCD, Almeida MS, Monteiro FR, et al. Assessment of the Interferon-Lambda-3 Polymorphism in the Antibody Response to COVID-19 in Older Adults Seropositive for CMV.Vaccines. 2023;11(2).
2022
Momesso Santos CM, Amaral JB, Rossi M, Vieria RP, Abad CCC, Bachi ALL. Metabolic and immune/inflammatory alterations induced by a triathlon under extreme conditions.Frontiers in sports and active living. 2022;4:915343. 10.3389/fspor.2022.915343.
Dos Santos JMB, do Amaral JB, França CN, Monteiro FR, Alvares-Saraiva AM, Kalil S, et al. Distinct Immunological Profiles Help in the Maintenance of Salivary Secretory IgA Production in Mild Symptoms COVID-19 Patients.Frontiers in immunology. 2022;13:890887. 10.3389/fimmu.2022.890887.
Longo PL, de Aquino RC, Ortiz SRM, de Oliveira RS, Gavioli A, do Amaral JB, et al. Effects of physical distancing by COVID-19 pandemic on diet quality, neurological and immunological markers, and fecal microbiota of Brazilian older women.Frontiers in nutrition. 2022;9:972100. 10.3389/fnut.2022.972100.
Pacheco VB, Nery G, Fernandes LL, Santana TV, Jimenez M, Borges L, et al. Salivary Proteome, Inflammatory, and NETosis Biomarkers in Older Adult Practitioners and Nonpractitioners of Physical Exercise.Oxidative medicine and cellular longevity. 2022;2022:3725056. 10.1155/2022/3725056.
Cezário K, Santos C, Filho CMA, Amirato GR, Paixão VD, Almeida EB, et al. Older Women Who Practiced Physical Exercises before the COVID-19 Pandemic Present Metabolic Alterations and Worsened Functional Physical Capacity after One Year of Social Isolation.Healthcare (Basel, Switzerland). 2022;10. 10.3390/healthcare10091736.
Santos JGO, Migueis DP, Amaral JBD, Bachi ALL, Boggi AC, Thamboo A, et al. Impact of SARS-CoV-2 on saliva: TNF-⍺, IL-6, IL-10, lactoferrin, lysozyme, IgG, IgA, and IgM.Journal of oral biosciences. 2022;64:108-13. 10.1016/j.job.2022.01.007.
Callera A, Callera F, Brito AA, Oliveira CR, Bachi ALL, Vieira RP. Evaluation of cytokine profile in the different phases of the autologous hematopoietic stem cell transplantation in patients with multiple myeloma.Transplant immunology. 2022;70:101513. 10.1016/j.trim.2021.101513.
Molina-Molina E, Furtado GE, Jones JG, Portincasa P, Vieira-Pedrosa A, Teixeira AM, et al. The advantages of physical exercise as a preventive strategy against NAFLD in postmenopausal women.European journal of clinical investigation. 2022; 52(3): e13731.
2021
Felismino ES, Santos JMB, Rossi M, Santos CAF, Durigon EL, Oliveira DBL, et al. Better Response to Influenza Virus Vaccination in Physically Trained Older Adults Is Associated With Reductions of Cytomegalovirus-Specific Immunoglobulins as Well as Improvements in the Inflammatory and CD8(+) T-Cell Profiles.Front Immunol. 2021; 12:713763.
Pezato R, Dassi C, Stamm AC, Voegels RL. New Nasopharyngeal Flap for Posterior Skull-Base Reconstruction: The Upper-Tongue Flap. Int Arch Otorhinolaryngol (EFirst). 2021.
França CN, Bachi ALL, Kosugi EM, Pezato R, Machado Santelli GM, Amaral JBD. Three-dimensional cell culture for the study of nasal polyps. Braz J Otorhinolaryngol. 2021.
Andrade MS, Ferrer CRL, Vancini RL, Nikolaidis PT, Knechtle B, Rosemann T, et al. The Effect of Muscle Strength on Marathon Race-Induced Muscle Soreness. International Journal of environmental research and public health. 2021; 18(21).
Silva EPD, Jr., Borges L, Bachi ALL, Hirabara SM, Lambertucci RH. L-arginine Improves Plasma Lipid Profile and Muscle Inflammatory Response in Trained Rats After High-Intense Exercise. Research quarterly for exercise and sport. 2021;92(1):82-90.
Pezato R, Gregório LL, Pérez-Novo C, Bezerra TFP, Kosugi EM. Montelukast Has no Impact on the Systemic Production of TGFβ-1 in Patients with Nasal Polyposis Associated with Aspirin Intolerance.Int Arch Otorhinolaryngol. 2021;25(1):e88-e91.
Balsalobre L, Figueiredo AB, Pezato R, Fujita RR. Effect of topical corticosteroids on nasal patency after acute positive airway pressure exposure.Braz J Otorhinolaryngol. 2021;87(3):326-32.
Furtado GE, Letieri RV, Caldo-Silva A, Sardão VA, Teixeira AM, de Barros MP, et al. Sustaining efficient immune functions with regular physical exercise in the COVID-19 era and beyond. European journal of clinical investigation. 2021;51(5):e13485.
Paixão V, Almeida EB, Amaral JB, Roseira T, Monteiro FR, Foster R, et al. Elderly Subjects Supplemented with L-Glutamine Shows an Improvement of Mucosal Immunity in the Upper Airways in Response to Influenza Virus Vaccination.Vaccines. 2021;9(2):107.
Cordeiro TG, Amaral JBd, Pavão V, Cardoso RG, Voegels RL, Pezato PM, et al. Fire simulator exposure alters the innate epithelial response and inflammatory status in the airways of firefighters.Rhinology. 2021;59(3):267-76.
Dos Santos JMB, Soares CP, Monteiro FR, Mello R, do Amaral JB, Aguiar AS, et al. In Nasal Mucosal Secretions, Distinct IFN and IgA Responses Are Found in Severe and Mild SARS-CoV-2 Infection.Front Immunol. 2021;12:595343.
Lucas BR, Voegels RL, do Amaral JB, Bachi ALL, Pezato R. BMP-7, MMP-9, and TGF-β tissue remodeling proteins and their correlations with interleukins 6 and 10 in chronic rhinosinusitis.Eur Arch Otorhinolaryngol. 2021.
Amirato GR, Borges JO, Marques DL, Santos JMB, Santos CAF, Andrade MS, et al. L-Glutamine Supplementation Enhances Strength and Power of Knee Muscles and Improves Glycemia Control and Plasma Redox Balance in Exercising Elderly Women. Nutrients. 2021;13(3).
Caldo-Silva A, Furtado GE, Chupel MU, Bachi ALL, de Barros MP, Neves R, et al. Effect of Training-Detraining Phases of Multicomponent Exercises and BCAA Supplementation on Inflammatory Markers and Albumin Levels in Frail Older Persons.Nutrients. 2021;13(4).
Lino WVS ; Bachi, A.L.B ; MENDES NETO, J. A. ; Caetani, G ; J.B. do Amaral, JB do Amaral ; PEZATO, R. CD133, a progenitor cell marker, is reduced in nasal polyposis and showed significant correlations with TGF-Beta1 and IL-8 Introduction.INTERNATIONAL ARCHIVES OF OTORHINOLARYNGOLOGY (PRINT), v. x, p. x, 2021.
Sperandio, A. ; Paixao, V. ; Almeida, E. ; J.b. do Amaral, JB do Amaral ; Pavão, , T.R.R ; Monteiro, F. ; Foster, R. ; Rossi m ; Amirato, G. ; Santos, C. ; Santos, J. M. B. ; Bachi, A.L.B. L-glutamine supplementation improves the inflammatory profile of exercised obese elderly.Revista Brasileira de Saúde Global, v. 2, p. 78-86, 2021.
2020
Coronel N, Pezato R, do Amaral JB, Ticlia L, Pinto Bezerra TF, Gomes LF, et al. Structural changes in the extracellular matrix after cross-linking of nasal polyp tissue. Acta otorhinolaryngologica Italica: organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale. 2020;40(6):426-34.
Bachi, A.L.B ; SANTOS, J. M. B. ; Monteiro, FR ; Rossi M ; J.B. do Amaral, JB do Amaral ; Soares CP ; Valerio AV ; Chalup VN ; Leite GF ; Almeida JA ; Safadi, MAP ; PAIATTO, B. N. M. ; Barrientos, A. C. M. ; Fevereiro, A. G. ; Paulis, M. ; Passadore, L. F. ; Ramalho, T. ; Vieira, R. P. ; Durigon, E. L. ; Oliveira, D. B.L. ; Penido , NO . Anosmia and ageusia in COVID-19 patients: the cytokines profile in upper airways.Revista Brasileira de Saúde Global, v. 1, p. 6-11, 2020.
Bettencourt, J. C. ; J.B. do Amaral, JB do Amaral ; Franca, C. N. ; Bachi, A. L. L. . Non-tumor cells role in the tumor microenvironment (TME) of head and neck cancer (HNC).Revista Brasileira de Saúde Global, v. 1, p. 16-20, 2020.
Cardoso CV, de Barros MP, Bachi ALL, Bernardi MM, Kirsten TB, de Fátima Monteiro Martins M, et al. Chemobrain in rats: Behavioral, morphological, oxidative and inflammatory effects of doxorubicin administration.Behavioural brain research. 2020;378:112233.
Pandolfi MM ; Machado, H. M. ; Franca, C. N. ; Higashi, L. S. F. ; Silva, M. V. L. ; Silva, L. F. G. ; Juliano, Y. ; do Amaral, J. B. ; Rodrigues, C. L. ; Souza, P. C. . Possíveis fatores associados à autopercepção da imagem corporal. REVISTA DE ATENÇÃO À SAÚDE, v. 18, p. 101, 2020.
El-Mafarjeh E, Martins GHC, Probst JJ, Santos-Dias A, Oliveira-Junior MC, de Barros MP, et al. Exercise Improves Lung Inflammation, but Not Lung Remodeling and Mechanics in a Model of Bleomycin-Induced Lung Fibrosis.Oxidative Medicine and Cellular Longevity. 2020;2020:4302608.
Monteiro FR, Roseira T, Amaral JB, Paixão V, Almeida EB, Foster R, et al. Combined Exercise Training and l-Glutamine Supplementation Enhances Both Humoral and Cellular Immune Responses after Influenza Virus Vaccination in Elderly Subjects.Vaccines. 2020;8(4).
Elias TGA, Monsanto RdC, do Amaral JB, Oyama LM, Maza PK, Penido NdO. Evaluation of Oxidative-Stress Pathway and Recovery of Sudden Sensorineural Hearing Loss.Int Arch Otorhinolaryngol. (EFirst).
De Paulis M, Oliveira DBL, Vieira RP, Pinto IC, Machado RRG, Cavalcanti MP, et al. Multisystem Inflammatory Syndrome Associated With COVID-19 With Neurologic Manifestations in a Child: A Brief Report.Pediatr Infect Dis J. 2020;39(10):e321-e4.
Fonseca F, Ballerini AP, Izar MC, Kato J, Ferreira CE, Fonzar W, et al. Advanced chronic kidney disease is associated with higher serum concentration of monocyte microparticles.Life Sciences. 2020;260:118295.
Aquino-Santos HC, Tavares-Vasconcelos JS, Brandão-Rangel MAR, Araújo-Rosa AC, Morais-Felix RT, Oliveira-Freitas S, et al. Chronic alteration of circadian rhythm is related to impaired lung function and immune response.International Journal of Clinical Practice. 2020;74(10):e13590.
Rodrigues JC, Bachi ALL, Silva GAV, Rossi M, do Amaral JB, Lezirovitz K, et al. New Insights on the Effect of TNF Alpha Blockade by Gene Silencing in Noise-Induced Hearing Loss.Int J Mol Sci. 2020;21(8).
Santos JdMBD, Bachi ALL, Luna Junior LA, Foster R, Sierra APR, Benetti M, et al. The Relationship of IL-8 and IL-10 Myokines and Performance in Male Marathon Runners Presenting Exercise-Induced Bronchoconstriction.International journal of environmental research and public health. 2020;17(8):2622.
Joffily L, Ungierowicz A, David AG, Melo B, Brito CLT, Mello L, et al. The close relationship between sudden loss of smell and COVID-19.Braz J Otorhinolaryngol. 2020;86(5):632-8.
Korn GP, Alvarenga EHL, Dall'Oglio GP, Azevedo RR, Chamun W, Cordeiro TG, et al. The Effects in the Upper Airway of Heat and Exposure to Combustion Byproducts of Burning Materials on Larynx of Firefighters.Journal of voice : official journal of the Voice Foundation. 2020.
2019
De Lira C, Vargas V, Silva W, Bachi A, Vancini R, Andrade M. Relative Strength, but Not Absolute Muscle Strength, Is Higher in Exercising Compared to Non-Exercising Older Women.Sports (Basel). 2019;7(1):19.
Foster R, Vaisberg M, Bachi ALL, dos Santos JdMB, de Paula Vieira R, Luna-Junior LA, et al. Premenstrual Syndrome, Inflammatory Status, and Mood States in Soccer Players.Neuroimmunomodulation. 2019;26(1):1-6.
Pezato R, Voegels RL, Pignatari S, Gregório LC, Pinto Bezerra TF, Gregorio L, et al. Nasal Polyposis: More than a Chronic Inflammatory Disorder - A Disease of Mechanical Dysfunction - The São Paulo Position.Int Arch Otorhinolaryngol. 2019 (EFirst).
Danella Figo D, De Amicis K, Neiva Santos de Aquino D, Pomiecinski F, Gadermaier G, Briza P, et al. Cashew Tree Pollen: An Unknown Source of IgE-Reactive Molecules.International Journal of Molecular Sciences. 2019;20(10):2397.
Almeida EB, Silva KPH, Paixão V, Amaral JBD, Rossi M, Xavier-Navarro RA, et al. A Mixture of Polyunsaturated Fatty Acids ω-3 and ω-6 Reduces Melanoma Growth by Inhibiting Inflammatory Mediators in the Murine Tumor Microenvironment.Int J Mol Sci. 2019;20(15).
Santos J, Foster R, Jonckheere AC, Rossi M, Luna Junior LA, Katekaru CM, et al. Outdoor Endurance Training with Air Pollutant Exposure Versus Sedentary Lifestyle: A Comparison of Airway Immune Responses. International journal of environmental research and public health. 2019;16(22).
do Amaral JB, Bloise AC, França CN, Perez-Novo C, Machado-Santelli GM, Alencar AM, et al. Alterations in cellular force parameters and cell projections in Nasal polyps-derived fibroblasts. Auris Nasus Larynx. 2019.
Garcia M, Santos-Dias A, Bachi ALL, Oliveira-Junior MC, Andrade-Souza AS, Ferreira SC, et al. Creatine supplementation impairs airway inflammation in an experimental model of asthma involving P2 × 7 receptor. Eur J Immunol. 2019;49(6):928-39.
Vaisberg M, Paixão V, Almeida EB, Santos JMB, Foster R, Rossi M, et al. Daily Intake of Fermented Milk Containing Lactobacillus casei Shirota (Lcs) Modulates Systemic and Upper Airways Immune/Inflammatory Responses in Marathon Runners.Nutrients. 2019;11(7).
Bachi ALL, Barros MP, Vieira RP, Rocha GA, de Andrade PBM, Victorino AB, et al. Combined Exercise Training Performed by Elderly Women Reduces Redox Indexes and Proinflammatory Cytokines Related to Atherogenesis.Oxid Med Cell Longev. 2019;2019:6469213.
Dos Santos CMM, Diniz VLS, Bachi ALL, Dos Santos de Oliveira LC, Ghazal T, Passos MEP, et al. Moderate physical exercise improves lymphocyte function in melanoma-bearing mice on a high-fat diet.Nutrition & metabolism. 2019;16:63.
Several studies have been carried out to clarify the etiopathogenesis of sensorineural hearing loss. There is no consensus, to date, on which factors are really important to predict the recovery of patients with this disease. Using different methodological approaches, the ORL Lab has described the role of different inflammatory cytokines and related cell signaling pathways in sensorineural hearing loss (More Information).
