O Laboratório de Neurociência tem a honra de convidar a todos para participar da aula “Next-generation miniscopes for recording neural activity during unrestrained behavior” que será ministrada pelo Prof. Dr.Tycho Hoogland, Professor Erasmus Medical Center, Dept. of Neuroscience, Rotterdam do Netherlands Institute for Neuroscience.
Data e horário: quinta-feira 09/08/2018 às 10:30h
Local Anfiteatro José Laredo Filho.
Dr. Hoogland is leading the development of novel miniscope imaging tools that have reduced size and weight opening up the possibility to study the multi-unit activity of neurons of different brain regions at the same time to study coherence between individual neurons in the cerebellum and those in the cerebral cortex of freely moving, awake behaving and sleeping animals in health and disease. Professor Hoogland also investigates the cellular basis of input integration in the inferior olive brain stem nucleus and its role in the timing and execution of complex movements.
Acesse para mais detalhes.
Abstract below sent by Dr.Hoogland:
The introduction of light-weight miniaturized fluorescence microscopes and recent open-source initiatives to disemminate the technology to a broader audience have permitted cellular-resolution population imaging during unrestrained, more natural animal behavior. We set out to develop a light-weight (1.6 g) open-source miniscope with a reduced footprint that incorporates a new imaging sensor, capability to monitor 3D-acceleration and permits remote optogenetic stimulation. With the footprint reduction we are able to record from two regions concurrently and demonstrate this by recording from cerebellum and cerebral cortex in freely behaving mice. We further demonstrate the functionality of the optogenetic probe by stimulating cerebral cortex, while recording in the cerebellum. Our new miniscope thus provides an integrated system for multi-region interrogation during unrestrained behaviors. In my talk I will give an overview of existing open-source miniscope technology including our own designs and potential applications in neuroscience.
Tycho Hoogland
Assistant Professor Erasmus MC, Dept. of Neuroscience, Rotterdam
Project lead next-generation miniscopes NIN-KNAW, Amsterdam